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KMID : 0387420080190010043
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Korean Society of Oriental Neuropsychiatry
2008 Volume.19 No. 1 p.43 ~ p.54
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Effect of Gamiheichumhwan Extract on the GABAergic Neurotransmission
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Seo Jong-Hoon
Kim Dong-Hyun
Lee Dong-Woong
Kim Geun-Woo
Koo Byung-Su
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Abstract
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Objective: The aim of this study is to evaluate the anticonvulsive effects of Gamiheichumhwan extract and to explain its action in GABAergic neuromodulation of the rat brain.
Method: The extracts of Gamiheichumhwan were investigated for their inhibitory effect on GABA transaminase activity, their influence on brain GABA and glutamate levels, their agonistic activity on GABA/benzodiazepine receptor and anticonvulsive action using in vitro and in vivo assays.
Results: 1. The extract inhibited dose-dependently GABA transaminase (GABA-T) activity by 4.6% and 18.9%, respectively at dosages of 250 mg/kg and 500mg/kg mouse (p.o.). 2. Brian GABA level was increased to 72.0% and brain glutamate level was decreased to 9.6% at a dosage of 500 mg/kg mouse (p.o.). 3. The extract suppressed [3H]Ro15-1788 binding to rat cerebral cortical membrane by at a dosage of 3.2 mg, suggesting its agonistic activity on GABA/benzodiazepin receptor. 4. The extract showed anticonvulsive effect by lengthening the onset time of convulsion, shortening the convulsion duration and diminishing the lethality.
Conclusion : It is suggested that Gamiheichumhwan can be used to somnipathy and adapted to treatment and prevention of epilepsy or convulsion.
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KEYWORD
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Gamiheichumhwan, Anticonvulsive Effect, GABA-transaminase, Neuromodulation, GABA, Glutamate
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